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About Onexio Biosystems

Onexio’s innovative microplate technologies allows co-culture and multiculture via micro-scale diffusion channels between adjacent wells. This patented design facilitates improved assay relevance in HTS. Established uses include integrating drug metabolism and other intercellular interactions in a technically simple format for drug/chemical safety and discovery applications. Our co-culture platform, the microDUO, is easily scaled (i.e., 96, 384, 1536-well plate formats and 48, 192 and 768 co-culture assays per plate respectively) in a ANSI/SLAS format that seamlessly integrates into existing automated HTS workflows. Co-culture can easily be initiated and reversed by the addition or removal of media, respectively. The wells in the microDUO plate can have clear or solid bottoms, allowing a range of assay endpoints on both cell types, (e.g., fluorescence, luminescence, or absorbance) to be measured by a standard plate reader or high-content imager. The microDUO offers a user-friendly assay set-up and while other technologies require extensive handling, equipment and unique expertise, the microDUO only requires the same level of operational knowledge needed for a standard microplate. The microDUO plate has been designed to be a cost-effective alternative to traditional co-culture platforms.

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